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Elucidating the behavior

Traffic with certain bursty characteristics can be qualitatively modeled by a DBMAP, as confirmed in [1].

designed GPMV isolation, microscopy and partitioning protocols; and E.

These observations are made in giant plasma membrane vesicles (GPMVs), which can be isolated by chemical vesiculants from a variety of cell types and microscopically observed using basic reagents and equipment available in any cell biology laboratory.

Microscopic phase separation is detectable by fluorescent labeling, followed by cooling of the membranes below their miscibility phase transition temperature.

This protocol describes the methods to prepare and isolate the vesicles, equipment to observe them under temperature-controlled conditions and three examples of fluorescence analysis: (i) fluorescence spectroscopy with an environment-sensitive dye (laurdan); (ii) two-photon microscopy of the same dye; and (iii) quantitative confocal microscopy to determine component partitioning between raft and nonraft phases.

GPMV preparation and isolation, including fluorescent labeling and observation, can be accomplished within 4 h.


  1. Elucidating the Role of the Paraventricular Nucleus of the Thalamus in Cue-Motivated Behavior by. Joshua Lewis Haight. A dissertation submitted in partial fulfillment of the requirements for the degree of. Doctor of Philosophy. Neuroscience in the University of Michigan. 2016. Doctoral Committee Assistant Professor.

  2. ELUCIDATING THE EVOLUTIONARY ORIGINS OF COLLECTIVE. ANIMAL BEHAVIOR. By. Randal S. Olson. Despite over a century of research, the evolutionary origins of collective animal behavior remain unclear. Dozens of hypotheses explaining the evolution of collective behavior have risen and fallen in the past.

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